Preparation of plant tissue to enable Spatial Transcriptomics profiling using barcoded microarrays.
Identifieur interne : 000D23 ( Main/Exploration ); précédent : 000D22; suivant : 000D24Preparation of plant tissue to enable Spatial Transcriptomics profiling using barcoded microarrays.
Auteurs : Stefania Giacomello [Suède] ; Joakim Lundeberg [Suède]Source :
- Nature protocols [ 1750-2799 ] ; 2018.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), ADN complémentaire (métabolisme), ADN des plantes (génétique), ADN des plantes (métabolisme), ARN des plantes (génétique), ARN des plantes (métabolisme), ARN messager (génétique), ARN messager (métabolisme), Analyse de profil d'expression de gènes (méthodes), Arabidopsis (croissance et développement), Arabidopsis (génétique), Arabidopsis (métabolisme), Codage à barres de l'ADN pour la taxonomie (méthodes), Feuilles de plante (croissance et développement), Feuilles de plante (génétique), Feuilles de plante (métabolisme), Fixation tissulaire (méthodes), Hybridation d'acides nucléiques (méthodes), Perméabilité (MeSH), Picea (croissance et développement), Picea (génétique), Picea (métabolisme), Populus (croissance et développement), Populus (génétique), Populus (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Séquençage par oligonucléotides en batterie (méthodes), Transcriptome (MeSH).
- MESH :
- croissance et développement : Arabidopsis, Feuilles de plante, Picea, Populus.
- génétique : ADN complémentaire, ADN des plantes, ARN des plantes, ARN messager, Arabidopsis, Feuilles de plante, Picea, Populus.
- métabolisme : ADN complémentaire, ADN des plantes, ARN des plantes, ARN messager, Arabidopsis, Feuilles de plante, Picea, Populus.
- méthodes : Analyse de profil d'expression de gènes, Codage à barres de l'ADN pour la taxonomie, Fixation tissulaire, Hybridation d'acides nucléiques, Séquençage par oligonucléotides en batterie.
- Perméabilité, Régulation de l'expression des gènes végétaux, Transcriptome.
English descriptors
- KwdEn :
- Arabidopsis (genetics), Arabidopsis (growth & development), Arabidopsis (metabolism), DNA Barcoding, Taxonomic (methods), DNA, Complementary (genetics), DNA, Complementary (metabolism), DNA, Plant (genetics), DNA, Plant (metabolism), Gene Expression Profiling (methods), Gene Expression Regulation, Plant (MeSH), Nucleic Acid Hybridization (methods), Oligonucleotide Array Sequence Analysis (methods), Permeability (MeSH), Picea (genetics), Picea (growth & development), Picea (metabolism), Plant Leaves (genetics), Plant Leaves (growth & development), Plant Leaves (metabolism), Populus (genetics), Populus (growth & development), Populus (metabolism), RNA, Messenger (genetics), RNA, Messenger (metabolism), RNA, Plant (genetics), RNA, Plant (metabolism), Tissue Fixation (methods), Transcriptome (MeSH).
- MESH :
- chemical , genetics : DNA, Complementary, DNA, Plant, RNA, Messenger, RNA, Plant.
- genetics : Arabidopsis, Picea, Plant Leaves, Populus.
- growth & development : Arabidopsis, Picea, Plant Leaves, Populus.
- metabolism : Arabidopsis, DNA, Complementary, DNA, Plant, Picea, Plant Leaves, Populus, RNA, Messenger, RNA, Plant.
- methods : DNA Barcoding, Taxonomic, Gene Expression Profiling, Nucleic Acid Hybridization, Oligonucleotide Array Sequence Analysis, Tissue Fixation.
- Gene Expression Regulation, Plant, Permeability, Transcriptome.
Abstract
Elucidation of the complex processes involved in plant growth requires analysis of the spatial gene expression patterns in all affected tissues. This protocol extension is an adaptation of a protocol that describes how to use barcoded oligo-dT microarrays to evaluate spatial global gene expression profiles in mammalian tissue to enable it to be applied to plant material. Here, we explain the required adjustments for preparing and treating plant tissue sections on the array surface, specifically in regard to how to permeabilize and remove the tissue. Once the tissue has been removed, the cDNA-mRNA hybrid that is left on the slide is processed in the same way as cDNA obtained during experiments on mammalian tissue; thus the later stages of the protocol are not included here, and readers should follow the accompanying protocol for those. We have previously used our protocol to generate high-quality sequencing libraries for Arabidopsis thaliana inflorescence, Populus tremula developing and dormant leaf buds, and Picea abies female cones. However, we anticipate that the protocol can be adapted to other tissue types and species. The entire protocol for preparing samples and processing libraries can be completed in 3-4 d.
DOI: 10.1038/s41596-018-0046-1
PubMed: 30353173
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Preparation of plant tissue to enable Spatial Transcriptomics profiling using barcoded microarrays.</title><author><name sortKey="Giacomello, Stefania" sort="Giacomello, Stefania" uniqKey="Giacomello S" first="Stefania" last="Giacomello">Stefania Giacomello</name><affiliation wicri:level="3"><nlm:affiliation>Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden. Stefania.Giacomello@scilifelab.se.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Science for Life Laboratory, National Bioinformatics Infrastructure Sweden, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden. Stefania.Giacomello@scilifelab.se.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Science for Life Laboratory, National Bioinformatics Infrastructure Sweden, Department of Biochemistry and Biophysics, Stockholm University, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName><orgName type="university">Université de Stockholm</orgName></affiliation></author><author><name sortKey="Lundeberg, Joakim" sort="Lundeberg, Joakim" uniqKey="Lundeberg J" first="Joakim" last="Lundeberg">Joakim Lundeberg</name><affiliation wicri:level="3"><nlm:affiliation>Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden. Joakim.Lundeberg@scilifelab.se.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:30353173</idno><idno type="pmid">30353173</idno><idno type="doi">10.1038/s41596-018-0046-1</idno><idno type="wicri:Area/Main/Corpus">000C05</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000C05</idno><idno type="wicri:Area/Main/Curation">000C05</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000C05</idno><idno type="wicri:Area/Main/Exploration">000C05</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Preparation of plant tissue to enable Spatial Transcriptomics profiling using barcoded microarrays.</title><author><name sortKey="Giacomello, Stefania" sort="Giacomello, Stefania" uniqKey="Giacomello S" first="Stefania" last="Giacomello">Stefania Giacomello</name><affiliation wicri:level="3"><nlm:affiliation>Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden. Stefania.Giacomello@scilifelab.se.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Science for Life Laboratory, National Bioinformatics Infrastructure Sweden, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden. Stefania.Giacomello@scilifelab.se.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Science for Life Laboratory, National Bioinformatics Infrastructure Sweden, Department of Biochemistry and Biophysics, Stockholm University, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName><orgName type="university">Université de Stockholm</orgName></affiliation></author><author><name sortKey="Lundeberg, Joakim" sort="Lundeberg, Joakim" uniqKey="Lundeberg J" first="Joakim" last="Lundeberg">Joakim Lundeberg</name><affiliation wicri:level="3"><nlm:affiliation>Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden. Joakim.Lundeberg@scilifelab.se.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author></analytic><series><title level="j">Nature protocols</title><idno type="eISSN">1750-2799</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (genetics)</term><term>Arabidopsis (growth & development)</term><term>Arabidopsis (metabolism)</term><term>DNA Barcoding, Taxonomic (methods)</term><term>DNA, Complementary (genetics)</term><term>DNA, Complementary (metabolism)</term><term>DNA, Plant (genetics)</term><term>DNA, Plant (metabolism)</term><term>Gene Expression Profiling (methods)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Nucleic Acid Hybridization (methods)</term><term>Oligonucleotide Array Sequence Analysis (methods)</term><term>Permeability (MeSH)</term><term>Picea (genetics)</term><term>Picea (growth & development)</term><term>Picea (metabolism)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (growth & development)</term><term>Plant Leaves (metabolism)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Populus (metabolism)</term><term>RNA, Messenger (genetics)</term><term>RNA, Messenger (metabolism)</term><term>RNA, Plant (genetics)</term><term>RNA, Plant (metabolism)</term><term>Tissue Fixation (methods)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>ADN complémentaire (métabolisme)</term><term>ADN des plantes (génétique)</term><term>ADN des plantes (métabolisme)</term><term>ARN des plantes (génétique)</term><term>ARN des plantes (métabolisme)</term><term>ARN messager (génétique)</term><term>ARN messager (métabolisme)</term><term>Analyse de profil d'expression de gènes (méthodes)</term><term>Arabidopsis (croissance et développement)</term><term>Arabidopsis (génétique)</term><term>Arabidopsis (métabolisme)</term><term>Codage à barres de l'ADN pour la taxonomie (méthodes)</term><term>Feuilles de plante (croissance et développement)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (métabolisme)</term><term>Fixation tissulaire (méthodes)</term><term>Hybridation d'acides nucléiques (méthodes)</term><term>Perméabilité (MeSH)</term><term>Picea (croissance et développement)</term><term>Picea (génétique)</term><term>Picea (métabolisme)</term><term>Populus (croissance et développement)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Séquençage par oligonucléotides en batterie (méthodes)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>DNA, Plant</term><term>RNA, Messenger</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Arabidopsis</term><term>Feuilles de plante</term><term>Picea</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term><term>Picea</term><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Arabidopsis</term><term>Picea</term><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>ADN des plantes</term><term>ARN des plantes</term><term>ARN messager</term><term>Arabidopsis</term><term>Feuilles de plante</term><term>Picea</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Arabidopsis</term><term>DNA, Complementary</term><term>DNA, Plant</term><term>Picea</term><term>Plant Leaves</term><term>Populus</term><term>RNA, Messenger</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>DNA Barcoding, Taxonomic</term><term>Gene Expression Profiling</term><term>Nucleic Acid Hybridization</term><term>Oligonucleotide Array Sequence Analysis</term><term>Tissue Fixation</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN complémentaire</term><term>ADN des plantes</term><term>ARN des plantes</term><term>ARN messager</term><term>Arabidopsis</term><term>Feuilles de plante</term><term>Picea</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Codage à barres de l'ADN pour la taxonomie</term><term>Fixation tissulaire</term><term>Hybridation d'acides nucléiques</term><term>Séquençage par oligonucléotides en batterie</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Permeability</term><term>Transcriptome</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Perméabilité</term><term>Régulation de l'expression des gènes végétaux</term><term>Transcriptome</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Elucidation of the complex processes involved in plant growth requires analysis of the spatial gene expression patterns in all affected tissues. This protocol extension is an adaptation of a protocol that describes how to use barcoded oligo-dT microarrays to evaluate spatial global gene expression profiles in mammalian tissue to enable it to be applied to plant material. Here, we explain the required adjustments for preparing and treating plant tissue sections on the array surface, specifically in regard to how to permeabilize and remove the tissue. Once the tissue has been removed, the cDNA-mRNA hybrid that is left on the slide is processed in the same way as cDNA obtained during experiments on mammalian tissue; thus the later stages of the protocol are not included here, and readers should follow the accompanying protocol for those. We have previously used our protocol to generate high-quality sequencing libraries for Arabidopsis thaliana inflorescence, Populus tremula developing and dormant leaf buds, and Picea abies female cones. However, we anticipate that the protocol can be adapted to other tissue types and species. The entire protocol for preparing samples and processing libraries can be completed in 3-4 d.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30353173</PMID><DateCompleted><Year>2019</Year><Month>04</Month><Day>17</Day></DateCompleted><DateRevised><Year>2019</Year><Month>10</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1750-2799</ISSN><JournalIssue CitedMedium="Internet"><Volume>13</Volume><Issue>11</Issue><PubDate><Year>2018</Year><Month>11</Month></PubDate></JournalIssue><Title>Nature protocols</Title><ISOAbbreviation>Nat Protoc</ISOAbbreviation></Journal><ArticleTitle>Preparation of plant tissue to enable Spatial Transcriptomics profiling using barcoded microarrays.</ArticleTitle><Pagination><MedlinePgn>2425-2446</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/s41596-018-0046-1</ELocationID><Abstract><AbstractText>Elucidation of the complex processes involved in plant growth requires analysis of the spatial gene expression patterns in all affected tissues. This protocol extension is an adaptation of a protocol that describes how to use barcoded oligo-dT microarrays to evaluate spatial global gene expression profiles in mammalian tissue to enable it to be applied to plant material. Here, we explain the required adjustments for preparing and treating plant tissue sections on the array surface, specifically in regard to how to permeabilize and remove the tissue. Once the tissue has been removed, the cDNA-mRNA hybrid that is left on the slide is processed in the same way as cDNA obtained during experiments on mammalian tissue; thus the later stages of the protocol are not included here, and readers should follow the accompanying protocol for those. We have previously used our protocol to generate high-quality sequencing libraries for Arabidopsis thaliana inflorescence, Populus tremula developing and dormant leaf buds, and Picea abies female cones. However, we anticipate that the protocol can be adapted to other tissue types and species. The entire protocol for preparing samples and processing libraries can be completed in 3-4 d.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Giacomello</LastName><ForeName>Stefania</ForeName><Initials>S</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-0738-1574</Identifier><AffiliationInfo><Affiliation>Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden. Stefania.Giacomello@scilifelab.se.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Science for Life Laboratory, National Bioinformatics Infrastructure Sweden, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden. Stefania.Giacomello@scilifelab.se.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lundeberg</LastName><ForeName>Joakim</ForeName><Initials>J</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-4313-1601</Identifier><AffiliationInfo><Affiliation>Science for Life Laboratory, Department of Gene Technology, KTH Royal Institute of Technology, Stockholm, Sweden. Joakim.Lundeberg@scilifelab.se.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Nat Protoc</MedlineTA><NlmUniqueID>101284307</NlmUniqueID><ISSNLinking>1750-2799</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012333">RNA, Messenger</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058893" MajorTopicYN="N">DNA Barcoding, Taxonomic</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009693" MajorTopicYN="N">Nucleic Acid Hybridization</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020411" MajorTopicYN="N">Oligonucleotide Array Sequence Analysis</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010539" MajorTopicYN="N">Permeability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D028222" MajorTopicYN="N">Picea</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012333" MajorTopicYN="N">RNA, Messenger</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016707" MajorTopicYN="N">Tissue Fixation</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059467" MajorTopicYN="Y">Transcriptome</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>10</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>4</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>10</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30353173</ArticleId><ArticleId IdType="doi">10.1038/s41596-018-0046-1</ArticleId><ArticleId IdType="pii">10.1038/s41596-018-0046-1</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country><region><li>Svealand</li></region><settlement><li>Stockholm</li></settlement><orgName><li>Université de Stockholm</li></orgName></list><tree><country name="Suède"><region name="Svealand"><name sortKey="Giacomello, Stefania" sort="Giacomello, Stefania" uniqKey="Giacomello S" first="Stefania" last="Giacomello">Stefania Giacomello</name></region><name sortKey="Giacomello, Stefania" sort="Giacomello, Stefania" uniqKey="Giacomello S" first="Stefania" last="Giacomello">Stefania Giacomello</name><name sortKey="Lundeberg, Joakim" sort="Lundeberg, Joakim" uniqKey="Lundeberg J" first="Joakim" last="Lundeberg">Joakim Lundeberg</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D23 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000D23 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:30353173
|texte= Preparation of plant tissue to enable Spatial Transcriptomics profiling using barcoded microarrays.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30353173" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |